Developing a multilingual protocol for marine eDNA sampling in the SeaInsights project

On January 24, 2025

In the scope of the Italia-Slovenija Interreg SeaInsights project, we are thrilled to announce the development of a practical protocol for collecting marine environmental DNA (eDNA) samples. This protocol is a key deliverable in advancing the project’s mission of promoting sustainable management and protection of marine biodiversity in the Upper Adriatic.

Why marine eDNA?

Environmental DNA is revolutionizing biodiversity monitoring. By analysing genetic material shed into the environment by marine organisms, eDNA allows scientists to gain insights into species presence, ecosystem health, and biodiversity patterns—all without invasive sampling techniques. This innovative tool plays a vital role in improving the understanding of the Adriatic Sea’s complex ecosystems.

Key features of the protocol

The SeaInsights Marine eDNA Sampling Protocol is the result of a collaborative effort to standardize methods across borders. This ensures data compatibility and reliability for robust marine biodiversity assessments. Some highlights of the protocol include:

  • Standardized sampling design: Covering 24 transects across the Upper Adriatic.
  • Detailed sampling instructions: Comprehensive guidance for seawater sampling, filter handling, and metadata collection to minimize contamination and maximize data quality.
  • Field negative controls: Protocols to ensure accuracy and reliability in eDNA sample collection.

Multilingual accessibility

To ensure inclusivity and usability, the protocol has been made available in English, Italian, and Slovenian. This multilingual approach reflects the commitment of the SeaInsights project to effective cross-border cooperation and the dissemination of scientific knowledge.

Applications and impact

The protocol serves as a blueprint for collecting and processing marine eDNA samples. Its applications go beyond SeaInsights, potentially setting a standard for other transnational efforts focused on marine biodiversity monitoring. With harmonized methodologies, the protocol facilitates:

  • Transboundary cooperation: Enhancing collaboration between Italian and Slovenian research teams.
  • Comprehensive biodiversity monitoring: Enabling consistent data collection for understanding the Upper Adriatic’s biodiversity.
  • Foundation for future research: Supporting future innovations in eDNA-based studies and marine management.

Acknowledgments

This deliverable is the product of dedication and expertise within the SeaInsights team, supported by the Interreg VI-A Italia-Slovenija Program. Special thanks go to all collaborators who contributed to this pivotal milestone.

Stay tuned for updates as we continue to unlock new insights into the Adriatic’s marine ecosystems. You can explore more about the SeaInsights project on the official website: SeaInsights Project.

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